ABSTRACT
Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57 percent of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71 percent) and six (86 percent) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.
Subject(s)
Animals , Antibodies, Helminth/blood , Antigens, Helminth/blood , Cysticercosis/veterinary , Swine Diseases/diagnosis , Taenia solium/isolation & purification , Cysticercosis/diagnosis , Cysticercosis/immunology , Cysticercosis/parasitology , Enzyme-Linked Immunosorbent Assay , Immunoblotting , Reproducibility of Results , Sensitivity and Specificity , Swine , Swine Diseases/immunology , Swine Diseases/parasitology , Taenia solium/immunologyABSTRACT
We have observed the seropositive rate of Taenia solium cysticercosis in residents at Nabo Village, Tiandong County, Guangxi Zhuang Autonomous Region, China by enzyme linked immunosorbent assay. The village had been found to be a relatively high endemic area of porcine cysticercosis among roaming pigs. Of 202 persons examined four males aged 15, 25, 35 and 41 year-old exhibited absorbance (abs) at 0.18, 0.20, 0.35 and 0.55, respectively. In addition, two females whose ages were 35 and 39 years revealed specific antibody levels of abs 0.26 and 0.41 in their sera. Overall positive rate among the people was 2.97%. All of these persons agreed that they had ingested the pork infected with T. solium metacestode (TsM), while history of proglottid discharge was not noticed from all of them. Three males and one female complained of intermittent headache. Our findings reinforced not only that the prevalence of cysticercosis might be related with roaming pigs infected with TsM but also that behavioral and environmental practices in local community constituted risk factors for transmission of the infection.
Subject(s)
Middle Aged , Male , Humans , Female , Child , Animals , Adult , Adolescent , Taenia solium/isolation & purification , Swine Diseases/parasitology , Swine , Seroepidemiologic Studies , Rural Population , Neurocysticercosis/epidemiology , Meat/parasitology , Immunoblotting , Enzyme-Linked Immunosorbent Assay , China/epidemiology , Antibodies, Helminth/bloodABSTRACT
AimTo investigate the immune effects of the genetic engineering vaccine against swine cysticercosis with the animal mode of kunming mouse. Method78 kunming mice were randomly divided into three groups: 1 )injection one times with the vaccine, 2)injection twice with the vaccine at intervals of ten days, 3)non-vaccination group. All the mice were infected with the vial hatched oncopheres by the tag vein at 20 days after the first vaccination,and were killed and dissected to look for swine cysticerci 63 days later. In order to know the immune response of the vaccine and the stateof parasites in the rats,the antigens and antibodies of swine cysticerci were detected by immune methods. ResultsThe vaccine was safety to all the vaccinated rats. The antibodies against swine cysticerci began to appear positive in some of the immunized mice at 7 days postvaccination,and they were all positive at 21 days. 65 cysticerci were found in the mice of the nonvaccination group,which lodged mainly in the chests. 2 and lcysticerci were respectively found in two rats of the first group and in one rat of the second group, their protective rates of cysticerci were 96.9％ and 98. 4％,respectively. ConclusionKunming mouse as the animal model of swine cysticercosis was stable and reliable,and the genetic engineering vaccine against swine cysticercosis was safety and efficiency.